Reporter
Part:BBa_K892003
Designed by: Gregory Rowe Group: iGEM12_Washington (2012-06-21)
OmpR promoter + LacI inverter driving red luciferase
OmpR promoter drives the the production of LacI. LacI represses lacI+pL hybrid promoter which controls the production of a luciferase. the LacI switches off the luciferase output. lacI+pL promoter inducable by IPTG
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 3555
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1281
Illegal BamHI site found at 2446 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2395
Illegal AgeI site found at 4097 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1827
Illegal SapI.rc site found at 3611
[edit]
Categories
Parameters
None |